Protease means the [Protein](or [Enzyme]) which degrades (poly)peptides into shorter peptide or [AminoAcid]s. Most frequent example is a digestion enzyme - chymotrypsin or trypsin.

Today, [Protease] is noticed as a drug for several [Virus] disease - [AIDS] , poliomyelitis or plant viral disease.

Protease is classified as nucleophile residue in the active site.

  1. SerineProtease

  2. AspartylProtease

  3. CysteineProtease

Cleavege site of each protease

Reagent no.

Reagent

Cleavage rule

1

Trypsin

C-term to K/R

2

Lys-C

C-term to K

3

Arg-C

C-term to R

4

Asp-N

N-term to D

5

V8-bicarb

C-term to E

6

V8-phosph

C-term to E/D

7

Chymotrypsin

C-term to F/W/Y/L/M

8

CNBr

C-term to M

SeeAlso ViralProtease

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